anti prkcq  (Cell Signaling Technology Inc)

Journal: Frontiers in Immunology

Article Title: Deciphering the causal association and underlying transcriptional mechanisms between telomere length and abdominal aortic aneurysm

doi: 10.3389/fimmu.2024.1438838

Figure Lengend Snippet: The validation of biomarkers in murine AAA model. (A) Representative general photos of murine in AAA group and sham group. The diameters of aortas were measured. (B) Immunohistochemistry of PRKCQ in aortas of AAA and sham group. Relative expression of PLCH2, PRKCQ, and SMG1 in aorta (C) and blood (D) . Students’ t test. *P<0.05; **P<0.01; ***P<0.001; ns, not significant.

Article Snippet: Immunohistochemistry staining was performed using the primary antibody against PRKCQ (ab230971; Abcam; USA).

Techniques: Immunohistochemistry, Expressing

Journal: Cell Death & Disease

Article Title: Human gastric cancer progression and stabilization of ATG2B through RNF5 binding facilitated by autophagy-associated CircDHX8

doi: 10.1038/s41419-024-06782-8

Figure Lengend Snippet: A qPCR analysis of circDHX8 with AGO2-RIP. B RIP analysis of the relative interplay between circDHX8 and ATG2B. C Overlapping analysis of differentially expressed genes (DEGs) between the circDHX8-OE and control groups with DEGs from CPTAC. D qPCR analysis of MYC, PPP1R15A, CDKN1A, RAB7A, FOS, ATG2B, and PRKCQ after circDHX8 overexpression. E Western blotting analysis of the protein levels of MYC, PPP1R15A, CDKN1A, RAB7A, FOS, ATG2B, and PRKCQ after circDHX8 overexpression. F Protein stability of ATG2B was assessed by western blotting. G The effect of circDHX8 and chloroquine on ATG2B protein levels was analyzed by western blotting. H Western blotting assays showed that circDHX8 could suppress the ubiquitination of ATG2B. I Colony-forming capacity of MKN45 and AGS cells. J Transwell assays for migration and invasion in MKN45 and AGS cells. * P < 0.05, ** P < 0.01 and *** P < 0.001.

Article Snippet: Next, 5% skim milk was enclosed at room temperature for 1 h before being incubated overnight with the following primary antibodies: anti-SQSTM1/p62 antibody (ab109012, Abcam, Cambridge, UK), anti-LC3B antibody (ab192890, Abcam), anti-GAPDH antibody (60004-1-Ig, Proteintech, Beijing, China), anti-Myc tag antibody (ab32, Abcam), anti-GADD34/PPP1R15A antibody (ab9869, Abcam), anti-CDKN1A/P21 antibody (ab109520), Abcam, anti-c-FOS antibody (ab208942, Abcam), anti-PRKCQ antibody (ab230972, Abcam), anti-RAB7 antibody (ab137029, Abcam), anti-ATG2B antibody (ab189934, Abcam), anti-Ubiquitin antibody (ab134953, Abcam), anti-RNF5 antibody (ab308066, Abcam), anti-SIRT1 antibody (ab110304, Abcam), and anti-Actin antibody (Cat# AA128, Beyotime, Shanghai, China).

Techniques: Over Expression, Western Blot, Migration

Journal: Cancer biology & therapy

Article Title: MiR-128-1-5p inhibits cell proliferation and induces cell apoptosis via targeting PRKCQ in colorectal cancer.

doi: 10.1080/15384047.2023.2226421

Figure Lengend Snippet: Figure 5. The effect of miR-128-1-5p on CRC growth in vivo. (a) Representative images of mice bearing tumors from vector and miR-128-1-5p groups (a). The tumor volume (b) growth curves after injections and the tumor weight (c) was measured after mice sacrificed. (b) Detection of the expression of miR-128-1-5p (a) and PRKCQ (b) by qRT-PCR. (c) Immunohistochemistry analysis of Ki-67, PRKCQ, caspase-3 and Bcl-2 protein levels in xenograft tumor tissues from above mice. Data are represented as mean ± SEM. **p < .01.

Article Snippet: The primary antibodies used in this study included: PRKCQ (Proteintech Group, Inc, IL, USA), Caspase-3 (CST), Bax (CST), Bcl-2 (CST), and GAPDH (Proteintech Group, Inc).

Techniques: In Vivo, Plasmid Preparation, Expressing, Quantitative RT-PCR, Immunohistochemistry